The Ausubel laboratory uses genetic, genomic, and chemical genetic approaches to: (1) elucidate the molecular basis of microbial pathogenesis in the bacterial pathogens Pseudomonas aeruginosa and Pseudomonas syringae and (2) identify the components of the signaling pathways involved in the host innate immune response in the plant Arabidopsis thaliana and the nematode Caenorhabditis elegans.
Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants.
Authors: Authors: Friedman AM, Long SR, Brown SE, Buikema WJ, Ausubel FM.
Gene
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Gene
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Directed transposon Tn5 mutagenesis and complementation analysis of Rhizobium meliloti symbiotic nitrogen fixation genes.
Molecular genetics of symbiotic nitrogen fixation.
ISRm1: A Rhizobium meliloti insertion sequence that transposes preferentially into nitrogen fixation genes.
Authors: Authors: Ruvkun GB, Long SR, Meade HM, van den Bos RC, Ausubel FM.
J Mol Appl Genet
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J Mol Appl Genet
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Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti induced by transposon Tn5 mutagenesis.
Authors: Authors: Meade HM, Long SR, Ruvkun GB, Brown SE, Ausubel FM.
J Bacteriol
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J Bacteriol
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Nucleotide sequence of the gene coding for the nitrogenase iron protein from Klebsiella pneumoniae.
Molecular genetic analysis of Klebsiella pneumoniae nitrogen-fixation (nif) genes.
Authors: Authors: Ausubel FM, Cannon FC.
Cold Spring Harb Symp Quant Biol
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Cold Spring Harb Symp Quant Biol
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The cloning and transposon Tn5 mutagenesis of the glnA region of Klebsiella pneumoniae: identification of glnR, a gene involved in the regulation of the nif and hut operons.
A general method for site-directed mutagenesis in prokaryotes.
Interspecies homology of nitrogenase genes.