CHURCH LAB, Full time research intern in microbiology (HMS)

Description

Harvard Medical School is currently seeking an experienced, motivated and well-organized individual to join as a full-time research intern to work on a cutting-edge microbiology research project. This is a one year, full-time hands-on research position working as a member of a dynamic team that includes graduate students and postdocs. The candidate should be comfortable and proficient with laboratory research and a strong team player. The candidate must have prior experience in microbiology and molecular biology laboratory techniques. Key skills include genetic manipulation of E.coli such as DNA transformation, plasmid extraction, PCR and cloning. Experience with next generation sequencing (illumine MiSeq) and sequence analysis tools (such as Geneious), as well as familiarity with computational tools (such as R or python) will be viewed highly favorably. The position involves utilization of microbiology techniques along with development of new experimental methods, so understanding of underlying fundamental principles is required.

Key Responsibilities

  •  Molecular biology techniques
    •  Perform PCR and analyze results.
    •  Design and perform cloning of plasmids and strains.
    •  Prepare samples and perform next generation sequencing.
    •  Perform sequence analysis.
  • • Genetic manipulations of E.coli
    • Perform plating and culturing.
    • Perform transformation and extraction of DNA.
  • • Data analysis
    •  Analysis of next gen sequencing data
    •  Analysis and presentation of experimental results

Qualifications

  • BS degree in biology, biochemistry or related discipline is required.
  •  Prior experience in a research laboratory (i.e beyond undergrad coursework) is required.
  •  Highly independent and result-oriented, able to efficiently execute experiments in a dynamic environment and critically evaluate experimental results.
  • Experience with next generation sequencing (illumina MiSeq) is a plus.
  •  Familiarity with computational tools (such as R or python) is a plus.
  •  Familiarity with experimental design and data analysis.
  •  Enthusiasm for learning and developing new skills and expertise.
  •  Hard working, able to cooperate with a team of researchers.
  •  Strong written and verbal communication skills.
  •  Organized and able to maintain precise records of work.

The official title of this position is “Visiting PostGrad Research Fellow”. Please note we do not provide Visa Sponsorship for employment purposes. In compliance with federal law, all persons hired will berequired to verify identity and eligibility to work in the United States and to complete the required employment eligibility verification form upon hire.

Please send CV to hmsmicrobe@gmail.com with subject line: “InternCV_first name_family name”

COLAIACOVO LAB, Postdoctoral fellow in Aging at Harvard Medical School

A postdoctoral fellow position is available immediately in the Department of Genetics at Harvard Medical School to study the biology of aging. The position will be funded by a 5-year R01 research grant entitled “Molecular mechanisms by which mild elevation of mitochondrial superoxide extends longevity” under the direction of Dr. Jeremy Van Raamsdonk, in Dr. Monica Colaiacovo’s lab. Dr. Van Raamsdonk, seeks to understand the aging process and to use that information to promote healthy aging and decrease the severity of age-onset neurodegenerative disease. The laboratory primarily uses C. elegans as a genetic model organism, prior to validating results in mouse models.

For a list of publications from Dr. Van Raamsdonk and the Van Raamsdonk lab please see: https://scholar.google.com/citations?user=WEaryu0AAAAJ&hl=en&oi=ao

Required Qualifications:

  • Ph.D. in a related field of biology (e.g. genetics, neuroscience, molecular biology)

  • Extensive experience working with C. elegans

  • Ability to generate transgenic worms from design to cloning to microinjection to integration

  • Previous experience with molecular biology techniques (DNA, RNA, protein)

  • Ability to work independently and as part of a team

  • Ability to lead a research project from design stages to data analysis, figure preparation and manuscript writing

  • A passion for scientific research

  • Strong organization and time management skills

  • Meticulous attention to detail

  • Eligible to work/train in the United States

Additional expertise that would be beneficial:

  • Experience with Crispr-Cas9 genome editing techniques

  • Experience and interest in designing experiments based on large scale bioinformatics outputs (ranking candidates, testing biological significance)

  • Knowledge of the aging research field

  • A strong understanding of genetics

  • Experience with other model organisms especially mammalian systems

Application Process:

Interested applicants should email a (1) cover letter, (2) curriculum vitae, and (3) the names and contact information for three references directly to jeremy_vanraamsdonk@hms.harvard.edu

 

CHURCH LAB, Postdoctoral Fellow Opportunity: Computational biology for ancient DNA editing

April 2018

The Church lab has a funded opportunity for a postdoctoral scientist to further the development of the lab’s efforts for mammoth de-extinction and also aging research. The scientist in this position will be responsible for devising and optimizing sample preparation and sequencing library generation protocols and acquiring, validating, and analyzing DNA and RNA sequencing data from edited samples for the mammoth and aging research. Beyond these specific projects working in collaboration with two postdoctoral fellows in the lab, this scientist is expected to become a leading resource within the Church lab on analyzing ‘omics’ methods generally, who will consult and collaborate with others both within and outside of the lab on best ways of conducting and developing these exciting projects. The ideal candidate will have a demonstrated record of scientific rigor, ingenuity, and peer-reviewed publications, and be well-organized, self-motivated, and able to work well both in teams and alone.  Candidates should have a strong background and experience in computational aspects of high-throughput DNA and RNA sequencing, use of high-throughput omics data in the context of systems and/or synthetic biology projects, and a strong understanding of statistics. Experimental background is a plus but not required. Good oral and written communication skills are required.

Please include the following information in your cover letter:

Are you legally authorized to work in the United States? Will you now or in the future require sponsorship for employment visa status?

Email your cover letter and CV with the following reference code in the subject line:

Ref# DNA2018_Apr

To:   Eriona_Hysolli@hms.harvard.edu

Have any references sent directly from your referee to the same email address.

April 2018

The Church lab has a funded opportunity for a postdoctoral scientist to further the development of the lab’s efforts for mammoth de-extinction and also aging research. The scientist in this position will be responsible for devising and optimizing sample preparation and sequencing library generation protocols and acquiring, validating, and analyzing DNA and RNA sequencing data from edited samples for the mammoth and aging research. Beyond these specific projects working in collaboration with two postdoctoral fellows in the lab, this scientist is expected to become a leading resource within the Church lab on analyzing ‘omics’ methods generally, who will consult and collaborate with others both within and outside of the lab on best ways of conducting and developing these exciting projects. The ideal candidate will have a demonstrated record of scientific rigor, ingenuity, and peer-reviewed publications, and be well-organized, self-motivated, and able to work well both in teams and alone.  Candidates should have a strong background and experience in computational aspects of high-throughput DNA and RNA sequencing, use of high-throughput omics data in the context of systems and/or synthetic biology projects, and a strong understanding of statistics. Experimental background is a plus but not required. Good oral and written communication skills are required.

Please include the following information in your cover letter:

Are you legally authorized to work in the United States? Will you now or in the future require sponsorship for employment visa status?

Email your cover letter and CV with the following reference code in the subject line:

Ref# DNA2018_Apr

To:   Eriona_Hysolli@hms.harvard.edu

Have any references sent directly from your referee to the same email address.

 

CHURCH LAB, Multiple Positions

April 2018

The Church lab has funded opportunities for postdoctoral scientists to advance two synthetic biology projects funded by the U.S. Department of Energy's (DOE) biofuel generation programs

Please answer the following questions below in your cover letter and include your CV and recommendations via email to church_lab_admin@hms.harvard.edu.

  1.  Are you legally authorized to work in the United States? Will you now or in the future require sponsorship for employment visa status?
  2.  What is your ideal scenario for a postdoc and beyond?

Postdoctoral Fellow in Translation Engineering:

The aim of one project is to conduct large-scale reengineering of the protein translation systems of E. coli strains whose genomes have been recoded so that one or more codons are unused, so that proteins containing multiple, distinct non-standard amino acids (NSAAs) (including D-amino acids) can be efficiently generated.  The scientist in this position will work with others in the lab to devise and implement ambitious strategies for developing, testing, demonstrating, and optimizing integrated sets of translation system components (such as tRNAs, amino-acyl tRNA synthetases, ribosomal proteins, rRNAs, and associated translation factors), that will operate together or in parallel in support of this goal.

Postdoctoral Fellow in Biosensor Development

The aim of the second project is use synthetic biology, structural biology, and protein engineering methods to alter existing proteins, or de novo design new ones, that respond efficiently and specifically in microbes to small molecules of potential importance or interest to DOE’s program.  The scientist in this position will leverage high-throughput computational biology, synthesis, and sequencing methods to generate and screen diverse libraries of biosensor proteins and optimize their products, in ways that either further advance techniques already developed or demonstrated by the lab or work towards new ones, and will also consider how NSAAs might be used in protein redesign. The scientists in either of these positions are expected to become leading resources within the Church lab on the general techniques and biology relevant to their projects, who will consult and work with others both within and outside of the lab on the best ways of leveraging these methods in related applications or towards other goals. The ideal candidates will have demonstrated records of scientific rigor, ingenuity, and peer-reviewed publications, and will be well-organized, self-motivated, and to work well both in teams and alone.  Candidates should be fast learners who have prior experience with sets of high throughput experimental and computational methods that are relevant to the positions for which they apply, should be familiar with high-throughput omics data analysis techniques, and should have a strong understanding of statistics.  Experience with technology development of new omics capabilities is highly desirable.  Excellent oral and written communication skills are required.
 

 

YANKER LAB, Multiple Positions

January 2018

Positions available to study gene regulation, epigenetics and the molecular biology of aging and neurodegenerative diseases.  Projects focus on iPS cells, C. elegans, animal models and the biology of genes involved in aging and Alzheimer's disease. For reference see: Nature 507:448, 2014, J. Cell Biol. 203:457, 2013, Mol. Cell 40:333, 2010, Nature 464:529, 2010, Nature 429:883, 2004. Experience in molecular biology is required.

Send CV, a brief description of research experience and names of 3 references to: Dr. Bruce Yankner by email to aharwick@genetics.med.harvard.edu

 


KIM LABAdministrative Associate II Job Description

August 2017

The Kim Lab in the Stem Cell Program at Boston Children’s Hospital is seeking an Administrative Associate II to support Dr. Carla Kim and her lab members. 

The Administrative Assistant II will be responsible for:

  • Providing administrative support to a principal investigator and her lab in the Stem Cell Program. This growing lab engages in stem cell biology and cancer research, with a focus on lung biology.
  • For the principal investigator, managing travel arrangements, maintaining financial records, overseeing grant submissions, managing calendar, managing lab projects, and providing customer service to her lab members.
  • Assisting with lab operations including the on boarding of new members and support of existing members.
  • Maintaining lab information including policies and procedures, databases and websites.

To qualify, you must have:

  • A high school level of educational development and six months of work experience. Bachelor’s degree is preferred.
  • Extensive experience with Microsoft suite of products, particularly Excel, Word and Outlook.
  • Initiative in improving operations within area/department, and the ability to assists in implementing changes.
  • No certification, registration or licensure required.

For more information or to apply to this position, please click here to access the Boston Children’s Hospital Career Site.  You may also contact Dr. Carla Kim directly at Carla.Kim@Childrens.Harvard.edu or 617-919-4865.