Frederick W. Alt

Frederick Alt, Ph.D.

Charles A. Janeway Professor of Pediatrics, Boston Children's Hospital
Professor of Genetics, Boston Children's Hospital
Charles A. Janeway Professor of Pediatrics and Genetics, Harvard Medical School
617-919-2539
Frederick Alt Website

The Alt laboratory studies mechanisms that maintain genomic stability in mammalian cells. The programmed recombination and hypermutation events in lymphocytes and the general DNA repair mechanisms involved in these processes are a focus. The laboratory also studies mechanisms that promote and prevent oncogenic translocations. Approaches range from molecular genetics and biochemistry to animal models.

Mapping the dynamic genetic regulatory architecture of HLA genes at single-cell resolution.
Authors: Authors: Kang JB, Shen AZ, Sakaue S, Luo Y, Gurajala S, Nathan A, Rumker L, Aguiar VRC, Valencia C, Lagattuta K, Zhang F, Jonsson AH, Yazar S, Alquicira-Hernandez J, Khalili H, Ananthakrishnan AN, Jagadeesh K, Dey K, Daly MJ, Xavier RJ, Donlin LT, Anolik JH, Powell JE, Rao DA, Brenner MB, Gutierrez-Arcelus M, Raychaudhuri S.
medRxiv
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Strategies for HIV-1 vaccines that induce broadly neutralizing antibodies.
Authors: Authors: Haynes BF, Wiehe K, Borrow P, Saunders KO, Korber B, Wagh K, McMichael AJ, Kelsoe G, Hahn BH, Alt F, Shaw GM.
Nat Rev Immunol
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Humanized V(D)J-rearranging and TdT-expressing mouse vaccine models with physiological HIV-1 broadly neutralizing antibody precursors.
Authors: Authors: Luo S, Jing C, Ye AY, Kratochvil S, Cottrell CA, Koo JH, Chapdelaine Williams A, Francisco LV, Batra H, Lamperti E, Kalyuzhniy O, Zhang Y, Barbieri A, Manis JP, Haynes BF, Schief WR, Batista FD, Tian M, Alt FW.
Proc Natl Acad Sci U S A
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Longitudinal analysis of invariant natural killer T cell activation reveals a cMAF-associated transcriptional state of NKT10 cells.
Authors: Authors: Kane H, LaMarche NM, Ní Scannail Á, Garza AE, Koay HF, Azad AI, Kunkenmoeller B, Stevens B, Brenner MB, Lynch L.
Elife
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Structural principles of B cell antigen receptor assembly.
Authors: Authors: Dong Y, Pi X, Bartels-Burgahn F, Saltukoglu D, Liang Z, Yang J, Alt FW, Reth M, Wu H.
Nature
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An antibody from single human VH-rearranging mouse neutralizes all SARS-CoV-2 variants through BA.5 by inhibiting membrane fusion.
Authors: Authors: Luo S, Zhang J, Kreutzberger AJB, Eaton A, Edwards RJ, Jing C, Dai HQ, Sempowski GD, Cronin K, Parks R, Ye AY, Mansouri K, Barr M, Pishesha N, Williams AC, Vieira Francisco L, Saminathan A, Peng H, Batra H, Bellusci L, Khurana S, Alam SM, Montefiori DC, Saunders KO, Tian M, Ploegh H, Kirchhausen T, Chen B, Haynes BF, Alt FW.
Sci Immunol
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The role of chromatin loop extrusion in antibody diversification.
Authors: Authors: Zhang Y, Zhang X, Dai HQ, Hu H, Alt FW.
Nat Rev Immunol
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SHLD1 is dispensable for 53BP1-dependent V(D)J recombination but critical for productive class switch recombination.
Authors: Authors: Vincendeau E, Wei W, Zhang X, Planchais C, Yu W, Lenden-Hasse H, Cokelaer T, Pipoli da Fonseca J, Mouquet H, Adams DJ, Alt FW, Jackson SP, Balmus G, Lescale C, Deriano L.
Nat Commun
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C-terminal deletion-induced condensation sequesters AID from IgH targets in immunodeficiency.
Authors: Authors: Xie X, Gan T, Rao B, Zhang W, Panchakshari RA, Yang D, Ji X, Cao Y, Alt FW, Meng FL, Hu J.
EMBO J
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mRNA-encoded HIV-1 Env trimer ferritin nanoparticles induce monoclonal antibodies that neutralize heterologous HIV-1 isolates in mice.
Authors: Authors: Mu Z, Wiehe K, Saunders KO, Henderson R, Cain DW, Parks R, Martik D, Mansouri K, Edwards RJ, Newman A, Lu X, Xia SM, Eaton A, Bonsignori M, Montefiori D, Han Q, Venkatayogi S, Evangelous T, Wang Y, Rountree W, Korber B, Wagh K, Tam Y, Barbosa C, Alam SM, Williams WB, Tian M, Alt FW, Pardi N, Weissman D, Haynes BF.
Cell Rep
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