The Ausubel laboratory uses genetic, genomic, and chemical genetic approaches to: (1) elucidate the molecular basis of microbial pathogenesis in the bacterial pathogens Pseudomonas aeruginosa and Pseudomonas syringae and (2) identify the components of the signaling pathways involved in the host innate immune response in the plant Arabidopsis thaliana and the nematode Caenorhabditis elegans.
Activation of Klebsiella pneumoniae and Rhizobium meliloti nitrogenase promoters by gln (ntr) regulatory proteins.
Authors: Authors: Sundaresan V, Ow DW, Ausubel FM.
Proc Natl Acad Sci U S A
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Proc Natl Acad Sci U S A
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Promoters regulated by the glnG (ntrC) and nifA gene products share a heptameric consensus sequence in the -15 region.
Authors: Authors: Ow DW, Sundaresan V, Rothstein DM, Brown SE, Ausubel FM.
Proc Natl Acad Sci U S A
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Proc Natl Acad Sci U S A
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Klebsiella pneumoniae nifA product activates the Rhizobium meliloti nitrogenase promoter.
Regulation of nitrogen metabolism genes by nifA gene product in Klebsiella pneumoniae.
The genetic engineering of nitrogen fixation.
Nitrogen fixation by Klebsiella pneumoniae is inhibited by certain multicopy hybrid nif plasmids.
Physical and genetic characterization of Rhizobium meliloti symbiotic mutants.
Authors: Authors: Buikema WJ, Long SR, Brown SE, van den Bos RC, Earl C, Ausubel FM.
J Mol Appl Genet
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J Mol Appl Genet
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Construction of a correlated physical and genetic map of the Klebsiella pneumoniae hisDGO region using transposon Tn5 mutagenesis.
Authors: Authors: de Bruijn FJ, Stroke IL, Marvel DJ, Ausubel FM.
EMBO J
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EMBO J
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Structural studies of alfalfa roots infected with nodulation mutants of Rhizobium meliloti.
Authors: Authors: Hirsch AM, Long SR, Bang M, Haskins N, Ausubel FM.
J Bacteriol
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J Bacteriol
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Directed transposon Tn5 mutagenesis and complementation analysis of Rhizobium meliloti symbiotic nitrogen fixation genes.